rabbit anti mannose receptor anti cd206 antibody  (Boster Bio)

Journal: Biomaterials Research

Article Title: Biomimetic Gradient Microporous Scaffold with a Triply Periodic Minimal Surface Enhances Osseointegration by Modulating Macrophage Polarization

doi: 10.34133/bmr.0266

Figure Lengend Snippet: In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor (CD206, M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Article Snippet: Rabbit anti-C-C motif chemokine receptor 7 (anti-CCR7) antibody (M1 marker, Boster, China) and rabbit anti-mannose receptor (anti-CD206) antibody (M2 marker, Boster, China) were used to stain the samples, as these are well-known markers for M1 and M2 macrophage phenotypes, respectively.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing

Journal: International immunopharmacology

Article Title: Minocycline ameliorates cognitive impairment in rats with trigeminal neuralgia by regulating microglial polarization.

doi: 10.1016/j.intimp.2024.113786

Figure Lengend Snippet: Fig. 6. Effect of minocycline on M1/M2 microglial polarization in TN rat hippocampi. (A-F) CD86, iNOS, Arg1, and CD206 protein levels were measured using Western blot analysis. Data are presented as mean ± SD (n = 3). ***P < 0.001/**P < 0.01/*P < 0.05 vs Sham; ##P < 0.01 vs dION-CCI.

Article Snippet: The primary antibodies used were against Neuronal nuclear antigen (NeuN) (ab60176, 1:1000, Abcam), Brain-derived neurotrophic factor (BDNF) (ab108319, 1:1000, Abcam), Tyrosine receptor kinase B (TrkB) (4603, 1:1000, Cell Signaling Technology, Danvers, MA, USA), Synaptophysin (SYP) (17785–1-AP, 1:6000, Proteintech Group, China), Postsynaptic density protein-95 (PSD95) (3409, 1:1000, Cell Signaling Technology), Iba1 (ab178846, 1:1000, Abcam), Cluster of Differentiation 86 (CD86) (sc-28347, 1:700, SantaCruz), iNOS (sc-7271, 1:500, SantaCruz), Arg1 (ab96183, 1:1000, Abcam), Mannose receptor (CD206) (24595, 1:1000, Cell Signaling Technology), TNF-α (AF7014, 1:500, Affinity, USA), IL-1β (AF5103, 1:1000, Affinity), IL-4 (sc-53084, 1:700, SantaCruz), IL-10 (DF6894, 1:1000, Affinity), TLR4 (sc-293072, 1:1000, SantaCruz), Myeloid differentiation primary response 88 (MyD88) (AF5195, 1:1000, Affinity), Nuclear factor-kappa B (NF-κb) (ABM0017, 1:1000, Abbkine, China), and β-actin (AF7018, 1:5000, Affinity).

Techniques: Western Blot

Journal: Heliyon

Article Title: M1 macrophages as promising agents for cell therapy of endometriosis

doi: 10.1016/j.heliyon.2024.e36340

Figure Lengend Snippet: Investigation of the macrophage phenotype in the formed foci of a syngeneic mouse model of endometriosis. A, B – In vivo live imaging of Cy7.5-labeled endometriosis foci formed in mice 3 weeks after transplantation: abdominal view – A , lateral view – B . C, D – Image of formed endometriosis foci in mice. White arrows indicate foci. The scale value is 1 mm. E, F – Section of an endometriosis foci stained with H&E. Scale bar C – 394 μ m, D – 202 μ m. G, H – Detection of E-cadherin by the HRP DAB substrate in epithelial cells of endometrium and of endometriosis foci, respectively. Nuclei are stained with hematoxylin. Red arrows indicate positively stained cells. Scale bar 50 μ m. I – Detection of CD86+ and CD206+ macrophages by HRP DAB substrate in endometrium and in foci of endometriosis. Nuclei are stained with hematoxylin. Red arrows indicate positively stained cells. Scale bar 50 μ m. J – Percentage of CD86+ and CD206+ macrophages in endometriosis foci compared to endometrium. K, L – Relative expression levels of M1 macrophage marker genes (K) and M2 macrophage marker genes (L) in endometriosis foci and in uterus. Expression levels were calculated using the 2-ΔCt method. The expression of each gene was normalized to the housekeeping gene GAPDH. M – Western blot analysis of Arginase 1, CD86 proteins in endometriosis foci and in uterus. Loading control GAPDH. N – Relative level of analyzed proteins in endometriosis foci and in uterus. Statistical analysis was carried out by non-parametric Mann–Whitney U test, *–р-value<0,05, **– p-value<0,005.

Article Snippet: The assay detected antigens by incubating sections with the following primary antibodies: goat anti-E-cadherin antibody (ab76319, Abcam, UK), rabbit anti-CD86 antibody (sc-9092, Santa Cruz Biotechnology, USA), rabbit anti-mannose receptor (CD206) antibody (ab64693, Abcam, UK), at a dilution of 1/200 each, overnight at +4 °C.

Techniques: In Vivo, Imaging, Labeling, Transplantation Assay, Staining, Expressing, Marker, Western Blot, Control, MANN-WHITNEY

Journal: Heliyon

Article Title: M1 macrophages as promising agents for cell therapy of endometriosis

doi: 10.1016/j.heliyon.2024.e36340

Figure Lengend Snippet: Analyzing the results of introducing M1 macrophages into a syngeneic model of endometriosis. А – Image of endometriosis foci in control mice and in mice after M1 macrophage therapy. White arrows indicate the foci. The scale value is 1 mm. B – Estimation of the number of the foci in mice after treatment compared with control mice. C – Detection of CD86+ and CD206+ macrophages by HRP DAB substrate in endometriosis foci. Nuclei are stained with hematoxylin. Red arrows indicate positively stained cells. The scale bar is 50 μ m. D – Percentages of CD86+ and CD206+ macrophages in the endometriosis foci. E, F – Relative expression levels in the endometriosis foci and the uterus of M1 macrophage (E) and M2 macrophage (F) marker genes. Expression levels were calculated as indicated in I and J . G – Western blot analysis of Arginase 1, CD206, SOCS3, CD86 proteins in endometriosis foci, control and after M1 macrophage therapy. Loading control GAPDH. H – Relative level of analyzed proteins in the endometriosis foci. Statistical analysis was carried out by non-parametric Mann–Whitney U test, *–р-value<0,05, **– p-value<0,005.

Article Snippet: The assay detected antigens by incubating sections with the following primary antibodies: goat anti-E-cadherin antibody (ab76319, Abcam, UK), rabbit anti-CD86 antibody (sc-9092, Santa Cruz Biotechnology, USA), rabbit anti-mannose receptor (CD206) antibody (ab64693, Abcam, UK), at a dilution of 1/200 each, overnight at +4 °C.

Techniques: Control, Staining, Expressing, Marker, Western Blot, MANN-WHITNEY

Journal: Heliyon

Article Title: M1 macrophages as promising agents for cell therapy of endometriosis

doi: 10.1016/j.heliyon.2024.e36340

Figure Lengend Snippet: Analysis of peritoneal macrophages from three groups of animals. Healthy mice (A) , mice with endometriosis (B) , mice after M1 macrophage therapy (C) : a', b', c' – isolation of the main pool of cells on FSC-SSC dot-plot; a'', b'', c'' – isolation of F4/80 hi CD11b hi (pink) and F4/80 lo CD11b lo (blue) subpopulations; a''', b''', c'''' – percentage of F4/80 hi CD11b hi and F4/80 lo CD11b lo in the pool of peritoneal macrophages; a'''', b'''', c'''' – histograms of fluorescence signal intensity distribution, detection of MHCII+, CD86+, Ly6C+, CD206+, CD163+ cells. Green contour is negative control, purple contour is stained sample. D – Percentage content of F4/80 hi CD11b hi in peritoneal fluid of three groups of mice. E − Percentage content of F4/80 lo CD11b lo in the peritoneal fluid of three groups of mice. F – Percentage of CD206+, CD163+, CD86+, MHCII+ and Ly6C+ cells in the peritoneal fluid of three groups of mice. Statistical analysis was carried out by non-parametric Mann–Whitney U test ( a’’’ , b’’’ , c’’’ ) and One-way ANOVA with Tukey's post hoc tests ( D – F ), *–р-value<0,05, **– p-value<0,005.

Article Snippet: The assay detected antigens by incubating sections with the following primary antibodies: goat anti-E-cadherin antibody (ab76319, Abcam, UK), rabbit anti-CD86 antibody (sc-9092, Santa Cruz Biotechnology, USA), rabbit anti-mannose receptor (CD206) antibody (ab64693, Abcam, UK), at a dilution of 1/200 each, overnight at +4 °C.

Techniques: Isolation, Fluorescence, Negative Control, Staining, MANN-WHITNEY